Publications

I. Bosch, H. de Puig, M. Hiley, M. Carré-Camps, F. Perdomo-Celis, C. F. Narváez, D. M. Salgado,D. Senthoor, M. O’Grady, E. Phillips, A. Durbin, D. Fandos, H. Miyazaki, C.- W. Yen, M. Gélvez-Ramírez, R. V. Warke, L. S. Ribeiro, M. M. Teixeira, R. P. Almeida, J. E. Muñóz-Medina, J. Ludert, M. L. Nogueira, T. E. Colombo, A. C. B. Terzian, P. T. Bozza, A. S. Calheiros, Y. R. Vieira, G. Barbosa-Lima, A. Vizzoni, J. Cerbino-Neto, F. A. Bozza, T. M. L. Souza, M. R. O. Trugilho, A. M. B. de Filippis, P. C. de Sequeira, E. T. A. Marques, T. Magalhaes, F. J. Díaz, B. N. Restrepo, K. Marín, S. Mattar, D. Olson, E. J. Asturias, M. Lucera, M. Singla, G. R. Medigeshi, N. de Bosch, J. Tam, J. Gómez-Márquez, C. Clavet, L. Villar, K. Hamad-Schifferli, L. Gehrke, Rapid antigen tests for dengue virus serotypes and Zika virus in patient serum. Sci. Transl. Med. 9, eaan1589 (2017).

Li, Yun, Julien Muffat, Attya Omer, Irene Bosch, Madeline A. Lancaster, Mriganka Sur, Lee Gehrke, Juergen A. Knoblich, and Rudolf Jaenisch. 2017. “Induction of Expansion and Folding in Human Cerebral Organoids.” Cell Stem Cell 20 (3): 385–96.e3. doi:10.1016/j.stem.2016.11.017.

Muffat, Julien, Yun Li, Attya Omer, Ann Durbin, Irene Bosch, Grisilda Bakiasi, Edward Richards, Aaron Meyer, Lee Gehrke, and Rudolf Jaenisch. 2017. “A Possible Role Of Microglia In Zika Virus Infection Of The Fetal Human Brain.” bioRxiv. doi:10.1101/142497.

Metsky, Hayden C., Christian B. Matranga, Shirlee Wohl, Stephen F. Schaffner, Catherine A. Freije, Sarah M. Winnicki, Kendra West, et al. 2017. “Zika Virus Evolution and Spread in the Americas.” Nature 546 (7658): 411–15. doi:10.1038/nature22402.

Tam, Justina O., Helena de Puig, Chun-Wan Yen, Irene Bosch, Jose Gómez-Márquez, Charles Clavet, Kimberly Hamad-Schifferli, and Lee Gehrke. 2017. “A Comparison of Nanoparticle-Antibody Conjugation Strategies in Sandwich Immunoassays.” Journal of Immunoassay & Immunochemistry 38 (4): 355–77. doi:10.1080/15321819.2016.1269338.

Sánchez-Purrà, Maria, Marc Carré-Camps, Helena de Puig, Irene Bosch, Lee Gehrke, and Kimberly Hamad-Schifferli. 2017. “Surface-Enhanced Raman Spectroscopy-Based Sandwich Immunoassays for Multiplexed Detection of Zika and Dengue Viral Biomarkers.” ACS Infectious Diseases, September. doi:10.1021/acsinfecdis.7b00110.

Durbin, Ann Fiegen, Chen Wang, Joseph Marcotrigiano, and Lee Gehrke. 2016. “RNAs Containing Modified Nucleotides Fail To Trigger RIG-I Conformational Changes for Innate Immune Signaling.” mBio 7 (5). doi:10.1128/mBio.00833-16.

Pardee, Keith, Alexander A. Green, Melissa K. Takahashi, Dana Braff, Guillaume Lambert, Jeong Wook Lee, Tom Ferrante, et al. 2016. “Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components.” Cell 165 (5): 1255–66. doi:10.1016/j.cell.2016.04.059.

Yen, C. W., H. de Puig, J. O. Tam, J. Gomez-Marquez, I. Bosch, K. Hamad-Schifferli, and L. Gehrke. 2015. “Multicolored Silver Nanoparticles for Multiplexed Disease Diagnostics: Distinguishing Dengue, Yellow Fever, and Ebola Viruses.” Lab on a Chip 15: 1638–41. doi:10.1039/c5lc00055f.

Schmidt DJ, Pickett BE, Camacho D, Comach G, Xhaja K, Lennon NJ, Rizzolo K, de Bosch N, Becerra A, Nogueira ML, Mondini A, da Silva EV, Vasconcelos PF, Munoz-Jordan JL, Santiago GA, Ocazionez R, Gehrke L, Lefkowitz EJ, Birren BW, Henn MR, Bosch I. A phylogenetic analysis using full-length viral genomes of South American dengue serotype 3 in consecutive Venezuelan outbreaks reveals a novel NS5 mutation. Infect Genet Evol. 2011;11(8):2011-2019.Schmidt 2011

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Gomila RC, Martin GW, Gehrke L. NF90 binds the dengue virus RNA 3′ terminus and is a positive regulator of dengue virus replication. PLoS One. 2011;6(2):e16687.

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Uzri D, Gehrke L. Nucleotide sequences and modifications that determine RIG-I/RNA binding and signaling activities. J Virol. 2009;83(9):4174-4184.

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Simon AE, Gehrke L. RNA conformational changes in the life cycles of RNA viruses, viroids, and virus-associated RNAs. Biochim Biophys Acta. 2009;1789(9-10):571-583.

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Gomila RC, Gehrke L. Biochemical approaches for characterizing RNA-protein complexes in preparation for high resolution structure analysis. Methods Mol Biol. 2008;451:279-291.

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Reichert VL, Choi M, Petrillo JE, Gehrke L. Alfalfa mosaic virus coat protein bridges RNA and RNA-dependent RNA polymerase in vitro. Virology. 2007;364(1):214-226.

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Boyce M, Scott F, Guogas LM, Gehrke L. Base-pairing potential identified by in vitro selection predicts the kinked RNA backbone observed in the crystal structure of the alfalfa mosaic virus RNA-coat protein complex. J Mol Recognit. 2006;19(1):68-78.

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Petrillo JE, Rocheleau G, Kelley-Clarke B, Gehrke L. Evaluation of the conformational switch model for alfalfa mosaic virus RNA replication. J Virol. 2005;79(9):5743-5751.

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Guogas LM, Laforest SM, Gehrke L. Coat protein activation of alfalfa mosaic virus replication is concentration dependent. J Virol. 2005;79(9):5752-5761.

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Rocheleau G, Petrillo J, Guogas L, Gehrke L. Degenerate in vitro genetic selection reveals mutations that diminish alfalfa mosaic virus RNA replication without affecting coat protein binding. J Virol. 2004;78(15):8036-8046.

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Laforest SM, Gehrke L. Spatial determinants of the alfalfa mosaic virus coat protein binding site. RNA. 2004;10(1):48-58.

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Guogas LM, Filman DJ, Hogle JM, Gehrke L. Cofolding organizes alfalfa mosaic virus RNA and coat protein for replication. Science. 2004;306(5704):2108-2111.

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Swanson M, Ansel-McKinney P, Houser-Scott F, Yusibov V, Loesch-Fries LS, Gehrke L. Viral Coat Protein Peptides with Limited Sequence Homology Bind Similar Domains of Alfalfa Mosaic Virus and Tobacco Streak Virus RNAs. J Virol. 1998;72(4):3227-3234.

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Ansel-McKinney P, Gehrke L. RNA determinants of a specific RNA-coat protein peptide interaction in alfalfa mosaic virus: conservation of homologous features in ilarvirus RNAs. J Molecular Biology. 1998;278:767-785.

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MacBeth KJ, Ro YT, Gehrke L, Patterson JL. Cleavage site mapping and substrate-specificity of Leishmaniavirus 2-1 capsid endoribonuclease activity. J Biochem (Tokyo). 1997;122(1):193-200.

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Houser-Scott F, Ansel-McKinney PA, Cai JM, Gehrke L. In vitro genetic selection analysis of alfalfa mosaic virus coat protein binding to 3′-terminal AUGC repeats. J Virology. 1997;71:2310-2319.

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Hann LE, Webb AC, Cai JM, Gehrke L. Identification of a competitive translation determinant in the 3′ untranslated region of alfalfa mosaic virus coat protein mRNA. Molecular and Cellular Biology. 1997;17:2005-2013.

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Ansel-McKinney P, Gehrke L. Footprinting RNA-Protein Complexes with Hydroxyl Radicals. In: Richter JD, editor. Analysis of mRNA Formation and Function. New York: Academic Press; 1997. p. 285-303.

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Rousseau D, Kaspar R, Rosenwald I, Gehrke L, Sonenberg N. Translation initiation of ornithine decarboxylase and nucleocytoplasmic transport of cyclin D1 mRNA are increased in cells overexpressing eukaryotic initiation factor 4E. Proc Natl Acad Sci USA. 1996;93(3):1065-1070.

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Ansel-McKinney P, Scott SW, Swanson M, Ge X, Gehrke L. A Plant Viral Coat Protein RNA-Binding Consensus Sequence Contains a Crucial Arginine. EMBO Journal. 1996;15:5077-5084.

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Rosenwald IB, Kaspar R, Rousseau D, Gehrke L, Leboulch P, Chen JJ, Schmidt EV, Sonenberg N, London IM. Eukaryotic translation initiation factor 4E regulates expression of cyclin D1 at transcriptional and post-transcriptional levels. J Biol Chem. 1995;270(36):21176-21180.

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Hann LE, Gehrke L. Messenger RNAs containing the unstructured 5′ leader sequence of alfalfa mosaic virus RNA 4 translate inefficiently in extracts prepared from poliovirus-infected HeLa cells. J Virology. 1995;69:4986-4993.

Gray ML, Burstein D, Lesperance LM, Gehrke L. Magnetization transfer in cartilage and its constituent macromolecules. Magnetic Resonance in Medicine. 1995;34:319-325.

Kaspar RL, Gehrke L. Peripheral blood mononuclear cells stimulated with C5a or lipopolysaccharide to synthesize equivalent levels of IL-1 beta mRNA show unequal IL-1 beta protein accumulation but similar polyribosome profiles. J Immunol. 1994;153(1):277-286.

Houser-Scott F, Baer ML, Liem KF, Jr., Cai JM, Gehrke L. Nucleotide sequence and structural determinants of specific binding of coat protein or coat protein peptides to the 3′ untranslated region of alfalfa mosaic virus RNA 4. J Virology. 1994;68:2194-2205.

Gehrke L, Hann LE, Kaspar RL, editors. Translational Control of Gene Expression. San Diego: Academic Press; 1994.

Chefalo PJ, Yang JM, Ramaiah KVA, Gehrke L, Chen JJ. Inhibition of protein synthesis in insect cells by baculovirus-expressed heme-regulated eIF-2 alpha kinase. J Biol Chem. 1994;269(41):25788-25794.

Baer M, Houser F, Loesch-Fries LS, Gehrke L. Specific RNA binding by Amino-terminal peptides of alfalfa mosaic virus coat protein. EMBO J. 1994;13:727-735.

Auron PE, Quigley GJ, Rosenwasser LJ, Gehrke L. Multiple amino acid substitutions suggest a structural basis for the separation of biological activity and receptor binding in a mutated human interleukin-1b protein. Biochemistry. 1992;31(29):6632-6638.

Webb AC, Bradley MK, Phelan S, Wu J, Gehrke L. Use of the polymerase chain reaction for rapid screening and assessment of the fidelity and purity of recombinant baculovirus clones. Biotechniques. 1991;11:512-519.

Tumer NE, Kaniewski W, Haley L, Gehrke L, Lodge JK, Sanders P. The Second Amino Acid of Alfalfa Mosaic Virus Coat Protein Is Critical for Coat Protein-Mediated Protection. Proc Natl Acad Sci USA. 1991;88(6):2331-2335.

Conca W, Auron PE, Aoun-Wathne M, Bennett N, Seckinger P, Welgus H, Goldring RS, Eisenberg SP, Dayer JM, Krane SM, Gehrke L. A human interleukin-1b mutein activates immediate early genes without stimulation of metalloproteinase expression. J Biol Chem. 1991;266:16265-16268.

Chen JJ, Throop MS, Gehrke L, Kuo I, Pal JK, Brodsky M, London IM. Molecular cloning of the cDNA of heme-regulated eukaryotic initiation factor 2 a-subunit kinase of rabbit reticulocytes: homology to yeast GCN2 protein kinase and human dsRNA-dependent eIF-2a kinase. Proc Natl Acad Sci USA. 1991;88:7729-7733.

Chen JJ, Throop MS, Gehrke L, Kuo I, Pal JK, Brodsky M, London IM. Cloning of the cDNA of the Heme-Regulated Eukaryotic Initiation Factor-2alpha (eIF-2alpha) Kinase of Rabbit Reticulocytes – Homology to Yeast GCN2 Protein Kinase and Human Double-Stranded-RNA-Dependent eIF-2alpha Kinase. Proc Natl Acad Sci USA. 1991;88(17):7729-7733.

Gehrke L, Jobling SA, Paik LSK, McDonald B, Rosenwasser LJ, Auron PE. A point mutation uncouples interleukin-1b biological activity and receptor binding. J Biol Chem. 1990;265:5922-5925.

Gehrke L, Jobling SA, editors. Untranslated leader sequences and enhanced messenger RNA translational efficiency. Series H: Cell Biology ed. Berlin: Springer-Verlag; 1990.

Chen JJ, Pal J, Petryshyn R, Kuo I, Yang JM, Throop MS, Gehrke L, London IM. Amino acid microsequencing of internal tryptic peptides of heme-regulated eukaryotic initiation factor 2a subunit kinase: Homology to protein kinases. Proc Natl Acad Sci USA. 1990;88:315-319.

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